Primara Agritech

Training


Training
Genewin offers a wide of range of fields for student training programs and projects.
  • Soil analysis – Estimation of nutrients present in soil and identification of deficiencies
  • Water analysis – Estimation of parameters in water
  • Leaf analysis - Estimation of nutrients present in soil and identification of diseases and deficiencies
  • Plant Tissue Culture techniques
  • Explant Initiation of plants through Tissue culture
  • Production of Biofertilizers using carriers
  • Formulation of Biopesticides against common plant pests

Module 1 - Basic molecular Biology techniques
  • Isolation of DNA
  • RNA extraction
  • Isolation of Genomic DNA
  • Agarose Gel Electrophoresis
  • DNA Purification by gel elution
  • Quantification of DNA and RNA by UV spectrophotometry
  • Determination of melting temperature
  • Restriction digestion of DNA

Module 2 - Plant Tissue Culture
  • Media and buffer preparations
  • Sterilization of explants
  • Micropropagation
  • Callus culture
  • Anther culture
  • Synthetic seed production and cell suspension
  • Plant genomic DNA isolation
  • Protoplast isolation

Module 3 - Cloning and Expression
  • Bacterial transformation
  • Restriction digestion of vector
  • Competent cell preparation
  • Screening for recombinants
  • Ligation
  • Gel elution of DNA
  • Preparation of Insert
  • Isolation of Plasmid Vector and insert DNA

Module 4 - Immunology
  • Ouchterlony Double diffusion
  • Radial immuno diffusion
  • Immuno electrophoresis
  • Rocket immuno electrophoresis
  • Counter current immuno electrophoresis
  • Latex agglutinations and haem agglutination
  • Dot Elisa
  • WIDAL

Module 5 - PCR based techniques
  • PCR amplification
  • PCR product amplification
  • RAPD and RFLP fingerprinting
  • Gene specific PCR
  • Restriction Mapping
  • Restriction Digestion of Genomic DNA
  • Sequencing of RNA and DNA

Module 6 - Blotting techniques
  • Immunoblotting
  • Southern Blotting
  • Northerrn Blotting
  • Western Blotting
  • DNA Probe labeling and hybridization

Module 7 - Bioprocess
  • Aerobic and Anaerobic fermentation
  • Secondary metabolite production
  • Biofuels

Module 8 - Bioanalytical techniques in enzymes
  • Extraction and purification of enzymes
  • Quantitative and qualitative analysis of enzymes
  • Enzyme kinetics
  • Enzyme immobilization
  • Assays of clinically important enzymes
  • SDS PAGE and Western Blotting
  • Chromatographic technique
  • Electrophoretic technique
  • Colorimetric technique qualitative analysis of compounds
  • Spectrophotometric and centrifugation techniques
  • Phytochemical analysis Quantitative analysis of compounds.

Wet lab-real time projects are also offered.

List Of Instruments
  • Name Of Instruments
  • Agarose Gel Electrophoresis (Submerged and Standard) 2D Gel Apparatus
  • Air Compressors
  • Analytical Balances – 5 Nos
  • Anemometer (For Air Flow Detection)
  • Atomic Absorption Spectrophotometer software
  • Autoclaves (500 litres per day)
  • Bacteriological Incubator
  • Bioreactor (25 Litres)
  • Biosafety Cabinets
  • BOD Incubator
  • Bunsen Burners
  • Calorimeters
  • COD Digestion Apparatus
  • Compound Microscopes
  • Cyclomixer
  • Deep freezer
  • Digital Colony Counter
  • Distillation Unit
  • DO meter
  • Double Distillation Unit
  • Dry Bath
  • Dry Blotting Apparatus
  • Eppendorf Centrifuge Table Top
  • Fermentor
  • Flame Photometer
  • Freeze Dryer
  • Fridge (3 Nos)
  • Growth Racks for Plant Tissue Culture
  • High Performance Liquid Chromatography (HPLC)
  • High Performance Soxhlet Apparatus
  • High Temperature Heating Mantles
  • Homogenizers
  • Hot Air Oven
  • Hot Plate
  • Humidifiers (For Controlled Growth)
  • Imaging Trinocullar Microscope
  • Incubator Shakers
  • Laminar Airflow (LAF for Plant Tissue Culture- 8x2)
  • Laminar Airflow (Microbiology – 1No)
  • Light Microscopes
  • LUX meter (For Light Measurement)
  • Microfiltration and Ultrafiltration
  • Micro Oven
  • Mixer
  • Muffle Furnace
  • Nephelometer / Turbidometer
  • PCR Workstation
  • pH meter
  • Quartz Micro Kjeldhal Unit
  • Refrigerated Centrifuge
  • Rotary Evaporator
  • SDS Apparatus
  • Smart PCR
  • Sonicator
  • TDS / EC meter
  • Thin Layer Chromatography Chambers
  • Tissue Culture Stands
  • UV Chamber
  • UV Transilluminator
  • UV Visible Spectrophotometer software
  • Vacuum Dessicators
  • Visible Spectrophotometer
  • Water Analyzer (All Parameters)
  • Water Bath